Sensitivity of field tests, serological and molecular techniques for Plum Pox Virus detection in various tissues
نویسندگان
چکیده
منابع مشابه
Serological characterization of Hungarian plum pox virus isolates.
Three Hungarian (no. 2, 4 and 9), and a Moldavian (K) plum pox virus isolates were compared with a characterized Spanish isolate (5.15) by RT-PCR, ELISA, dot-blot and Western blot analysis. Monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the Spanish isolate were able to differentiate the four isolates. Hungarian isolate No. 2 prove...
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The present study was conducted on sixty healthy female domestic ruminants that suffered from still births or abortion, to find sero-positive cases and also to determine the probable role of uterine tissue in diagnosis of akabane viral infection and viral persistency. Because of this, a serological test (ELISA) and molecular (RT-PCR) diagnostic methods were used to find the seropositive cases a...
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Article available on line / Article disponible en ligne à l’adresse : -------------------------------------------------------------------------------------------------------------------------------------------------------------------------http://om.ciheam.org/article.php?IDPDF=3001769 ---------------------------------------------------------------------------------------------------------------...
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Plum pox virus (PPV) is the most damaging viral pathogen of stone fruits. The detection and identification of its strains are therefore of critical importance to plant quarantine and certification programs. Existing methods to screen strains of PPV suffer from significant limitations such as the simultaneous detection and genotyping of several strains of PPV in samples infected with different i...
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Article available on line / Article disponible en ligne à l’adresse : -------------------------------------------------------------------------------------------------------------------------------------------------------------------------http://om.ciheam.org/article.php?IDPDF=3001774 ---------------------------------------------------------------------------------------------------------------...
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ژورنال
عنوان ژورنال: Acta agriculturae Slovenica
سال: 2014
ISSN: 1581-9175,1854-1941
DOI: 10.14720/aas.2014.103.1.13